For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...

Another run with 12 samples was performed on the 32-well block during which a temperature of 99°C was maintained on the 64-well block. Results and discussion (1) PCR optimization via gradient function ...

Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...

Promega Corporation introduces Promega PCR Optimization Kit that allows customers to rapidly define their own unique PCR master mix manufactured under cGMP for research or clinical use. The user’s ...

The 2X PCR Master Mix is a made-to-order custom master mix. Once your order is received, your custom master mix will be delivered in 10 business days. The 2X formulation contains customer defined ...

Common problems that arise during PCR assays include weak target-binding, competing target secondary structure, primer-dimer formation, and mishybridization of PCR probes and primers. These issues, ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...